Morpho-biometrical, nutritional and phytochemical characterization of carrot landraces from Puglia region (southern Italy)

The explorations as a part of the regional BiodiverSO Programme of vegetable genetic resource rescue revealed that in the arenili (sandy shores) of “Salterns of Margherita di Savoia” (SMS), a coastal landscape area of Puglia region (southern Italy), along the commercial genotypes of small rooting species, landraces are still cultivated. The morpho-biometrical, nutritional and phytochemical properties of two carrot landraces (“Carota a punta lunga” and “Carota a punta tonda”) and a commercial carrot hybrid (“Presto”) collected from the SMS area are examined. The study highlighted that the pedological conditions of the arenili of the SMS area are the main driving force in controlling the nutritional and nutraceutical characteristics of carrot, conferring to genotypes grown in this area a high profile in comparison with literature data. In the site of cultivation of arenili, a large variability in the morpho-qualitative traits emerged among carrot genotypes. “Carota a punta tonda” stands for a promising genotype being very rich in phenols (209.8 mg kg-1 fw) (mainly di-caffeic acid derivative and chlorogenic acid), Β-carotene (21,512 µg 100 g-1 fw), and high antioxidative proprieties. “Carota a punta tonda” could be considered as a healthy product for consumers and also amenable to selection for breeding purpose. Increasing the knowledge about nutritional and nutraceutical properties of local landraces may push the preference of consumers beyond the local community and, at the same time, farmers can be stimulated to continue their cultivation. Thus, the promotion of their on-farm/in situ conservation (cultivation) could represent an efficient strategy for agro-biodiversity preservation

Nutritional characterization of two rare landraces of Turnip (Brassica rapa. var. rapa) tops and their on-farm conservation in Foggia Province

The study of nutritional properties in landrace products and the general context of its cultivation site are crucial to designing a sustainable on-farm strategy for landrace conservation. The present study describes the main nutritional aspects of two Brassica rapa subspecies rapa landraces collected in Puglia, Italy along with agroecological and socioeconomical traits where they are cultivated. The two B. rapa landraces ('Cima di rapa dalla testa' and 'Cima di rapa antica') are only found in sites at 700-800 m asl and in two landscape units (the Southern Daunian Mountains (SDM) and the Umbra Forest (UF), respectively) of the Foggia province. These rare landraces were selected by farmers to produce turnip greens/tops from ancient root turnip crops. They are named and consumed by local people in the same way as turnip tops of Brassica rapa subspecies sylvestris ('Cima di rapa'), which are widely cultivated in Puglia. Compared to the most common 'Cima di rapa', the two highlighted landraces have a better nutritional profile linked to an improved content in antioxidant compounds-i.e., vitamin C (458 mg kg-1 FW), total phenols (347 mg ac. gallic equivalent kg-1 FW)-in glucosinolate (741 μmol kg FW-1, in 'Cima di rapa antica') and in minerals, such as K. Both landraces are deemed as having a high risk of erosion. Few exemplars are cultivated in marginal lands and urban/peri-urban areas (SDM), or in isolated sites within the UF, which is a special protection zone within Gargano National Park. However, natural, cultural, and recreational tourism are the main economic activities in both landscape units

Evaluation of Garlic Landraces from Foggia Province (Puglia Region; Italy)

Interest in local landraces has unfortunately decreased over, the last decades, in which they have been continuously subjected to a high genetic erosion in favour of new modern varieties. Within the Puglia region (S-E Italy), Foggia province was found to be the richest in vegetable landraces. In the present study, six garlic landraces collected from this area have been assessed for their chemical composition (minerals, organic acids, free sugars, volatile, and phenolic compounds) along with their main morpho-biometrical traits. A commercial genotype was also considered as a reference standard. The landraces show a large variability, but in general high morphological standards, high levels of cations and phenols, and low levels of volatile-(S)-compounds in comparison with the commercial genotype and the literature values. 'Aglio di Peschici' and 'Aglio Rosso di Monteleone di Puglia' are very rich in minerals and phenols (mainly ferulic acid and iso-rhamnetin). This increase in knowledge on the chemical properties of these garlic landraces could represent a tool for encouraging the consumption of a food product. At the same time, the consumption of these landraces would stimulate their cultivation and could highly contribute to protection against the risk of erosion of agro-biodiversity by their in situ/on-farm conservation

Assessing Medical Students’ Self-Perceived Preparedness to Care for Gender Diverse Patients: A Survey Study

Background: Many transgender patients report avoiding healthcare due to discrimination, lack of provider knowledge, and perceived lower quality of care related to their gender identity. One factor contributing to these disparities may be a lack of preparation in medical school related to gender diversity. Methods: This cross-sectional survey study assessed third- and fourth-year medical students’ self-perceived preparedness to provide medical care for gender diverse patients, at one medical school in the United States. Mixed methods were used with both quantitative analyses and qualitative analyses using grounded theory. Results: 54 of 216 eligible students completed the survey (response rate 25%). 53.7% rated themselves as prepared to take a complete medical history from transgender patients compared to 94.4% for cisgender patients. 51.9% rated themselves as prepared to discuss cervical cancer screening with transmasculine patients. Only 31.5% rated themselves as prepared to provide inclusive preconception counselling. Concerns included using the wrong language and lacking appropriate medical knowledge. The most cited sources of learning about gender diversity were independent learning and fellow students. Five themes emerged in qualitative analyses, two of which included a request for greater opportunities to practice working with gender diverse patients and longitudinal integration of transgender medicine across the curriculum. Conclusion: Medical students who completed this survey rated themselves as less prepared to care for gender diverse patients compared to cisgender patients. Their current knowledge was largely based on learning outside of the medical school curriculum. The respondents highlighted many opportunities for improvement in medical school curricula

Aluminum strand coating for increasing the interstrand contact resistance in Rutherford type superconducting cables

The interstrand contact resistance (Rc) in Rutherford type cables for fast cycling superconducting magnets must be sufficiently high in order to limit eddy current losses. The required value for Rc depends on the cable and magnet geometries and on the foreseen cycling rate, but is typically of the order of one mW. Such values can be reached with a dedicated strand coating or with a resistive internal cable barrier. As a possible candidate Al strand coatings have been tested. For a Rutherford type inner conductor cable of the Large Hadron Collider (LHC) made of Al coated strands Rc values higher than 500 Omega are achieved. The native Al2O3 oxide layer formed at ambient temperature in air is sufficient to reach this high contact resistance. A 6 h-200 °C oxidation heat treatment in air with 100% relative humidity further increases Rc to values above 600 μOmega . Due to the high thermal and mechanical stability of Al2O3 only a relatively moderate Rc drop of about 40 % is obtained during a 190 °C heat treatment under 50 MPa pressure (the so-called curing cycle of the coil insulation) subsequent to the 6 h-200 °C oxidation heat treatment

B Cell Activation and Escape of Tolerance Checkpoints:Recent Insights from Studying Autoreactive B Cells

Autoreactive B cells are key drivers of pathogenic processes in autoimmune diseases by the production of autoantibodies, secretion of cytokines, and presentation of autoantigens to T cells. However, the mechanisms that underlie the development of autoreactive B cells are not well understood. Here, we review recent studies leveraging novel techniques to identify and characterize (auto)antigen-specific B cells. The insights gained from such studies pertaining to the mechanisms involved in the escape of tolerance checkpoints and the activation of autoreactive B cells are discussed. In addition, we briefly highlight potential therapeutic strategies to target and eliminate autoreactive B cells in autoimmune diseases